Recent studies have shown that selected active ingredients can be examined for effects of a cosmetic product on dermal extracellular functions. The results of one research were able to provide a clue on the effects of selected plant extracts contained in a cosmetic cream on extracellular components when viewed over a period of 3 months.
A study group of women of Caucasian descent between the ages 45 -65, were asked to randomly use a cosmetic product B with active components of jasmine and soy twice daily on one arm and a second cosmetic product A without the active ingredients of cosmetic product B for a three months period.
The Effects of a cosmetic product on dermal extracellular were reviewed by ensuring that measurements were taken of both arms before administering the treatment, as well as after the 4th and 12th week respectively from commencement of use.
The measurements used were a multiphoton tomography with deep cells resolution to obtain multi-layer images. The optical depth was 6 microns for a range of 0 to 200 microns based on two sets of wavelengths of 760nm and 820 nm.
To obtain objective determination of the second harmonic generation signal, compare series of images and auto-florescence, the approach ensured that the image integrated brightness was taken as a measure of the signal, with same dimensions for all image acquisitions.
The ratio of brightness of images treated with cosmetic A or B and untreated areas was calculated and used as the determination of the efficacy of treatment. The parameter deployed for statistical variance analysis was the difference in effect prior to and after 12 consecutive weeks of treatment with cosmetic A or B.
The extracellular background signals are not altered after 0, 4 and the 12th week when cosmetic A (with no active ingredients) was used. The use of cosmetic B resulted in an improved extracellular background signal by the 12th week. The comparison made between both wavelength segments, showed a remarkable increase in the deeper areas than the superficial segments following cosmetic B usage. There were no recorded changes for cosmetic A.
The use of multiphoton tomography was an inroad to images with excellent high-resolution, which provided a clear layout of the single cells, extracellular background and skin layers amongst the 24 volunteers used for the study.
The real effects of the statistical analysis showed the marked improvement from use of Cosmetic B with the plant extracts renowned for collagen synthesis. The observed changes were consistent with the nature of dermal collagen and elastin content modifications, which formed the basis for the examination of Effects of a cosmetic product on dermal extracellular